Characterization of B-Glucan from Oyster Mushroom (Pleurotus pulmonarius)
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Abstract
Mushrooms are known as B-glucan sources. The purpose of this investigation was to characterize and purify B-glucan by alkaline extraction from oyster mushrooms (Pleurotus pulmonarius) to evaluate its molecular weight (Mw) and viscosity. The ideal extraction process parameters were found as 80oC, 90 min, and 30% KOH. Fourier transforms-infrared spectroscopy analysis and 1H and 13C nuclear magnetic resonance spectroscopy were used for chemical characterization, and extracted B-glucan was found to be a B-(1-3) glucose polymer with B-(1-6) side chains. The Mw was determined as 349.26
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